Reactions of tubulin-associated guanine nucleotides.
نویسندگان
چکیده
منابع مشابه
Interactions of taxol, microtubule-associated proteins, and guanine nucleotides in tubulin polymerization.
Microtubule-associated proteins (MAPs), guanine nucleotides, and reaction temperature have substantial effects on the interaction of taxol with purified tubulin in 0.1 M glutamate. Without MAPs, taxol induced tubulin to polymerize only if GTP was present and the reaction mixture warmed. GTP was hydrolyzed in tandem with polymerization in stoichiometric amounts, while GDP inhibited both polymeri...
متن کاملGuanine Nucleotides Induce Ca2+-independent Insulin Secretion
The role of guanine nucleotides in insulin secretion was investigated in electrically permeabilized RINm5F cells. Ca2+ stimulated insulin release (EGO 2 p~ Ca2+). The GTP stable analog, GTPrS, elicited insulin secretion at vanishingly low Ca2+ concentrations (<lo-” M), slightly potentiated the response to intermediate Ca2+ levels, but exerted less than additive effects at maximal Ca2+ conce...
متن کاملRegulation of acetyl-CoA carboxylase by guanine nucleotides.
Acetyl-CoA carboxylase, a major rate-limiting enzyme for fatty acid synthesis, is subject to acute regulation by both allosteric modulation and covalent enzyme phosphorylation. Because citrate activation of the enzyme in vitro requires citrate concentrations far in excess of intracellular levels, we have attempted to identify other ligands which might mediate carboxylase activity. Heated liver ...
متن کاملThe interactions of cell division protein FtsZ with guanine nucleotides.
Prokaryotic cell division protein FtsZ, an assembling GTPase, directs the formation of the septosome between daughter cells. FtsZ is an attractive target for the development of new antibiotics. Assembly dynamics of FtsZ is regulated by the binding, hydrolysis, and exchange of GTP. We have determined the energetics of nucleotide binding to model apoFtsZ from Methanococcus jannaschii and studied ...
متن کاملGuanine nucleotides stimulate NADPH oxidase in membranes of human neutrophils.
In the chain of events by which chemotactic peptides stimulate NADPH oxidase-catalyzed superoxide formation in human neutrophils, the involvements of a pertussis toxin-sensitive guanine nucleotide-binding protein (N-protein), mobilization of intracellular calcium and protein kinase C stimulation have been proposed. Superoxide formation was studied in membranes from human neutrophils; NADPH oxid...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1978
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)62344-6